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- Posts: 26
- Joined: Wed Jun 05, 2019 3:57 pm
I have a method for aldehydes and ketones using DNPH with HPLC-DAD. Our formaldehyde method works great, but I can't seem to get Acetone to work.
I have tried 1-20ppm curves and also 20-50ppm curves. The peak area that shows up for Acetone increases slightly with increasing concentration, but it's nowhere near linear (1ppm gave 7 area and 20ppm gave 22).
My mobile phase is 60% Water : 40% Acetonitrile using a C18 column.
Reaction conditions: 1ml DNPH in 50ml acetontrile to give stock solution. 2.5ml of stock solution + 2.5ml acetone standard reacted for 30 minutes at room temperature.
Does anybody have any experience with Acetone detection with HPLC? All of the literature I can find says acetone reacts readily with DNPH.
Thanks