Hi everyone,

I would like to normalize the peak area of an analyte (A) using an internal standard (STD).

The internal standard used is not "similar" to my analyte and was used only for the purpose of normalizing the analyte peak areas in samples that were measured over time.

I don't want to use the formula A area / STD area because I don't want to affect the absolute peak A intensity.

I think that in this case the best formula to use could be the following:

Normalized area = Analyte area x Scaling factor

where the scaling factor is defined as:

Scaling factor = mean of STD Area/Area of STD in sample (among all samples)

It could be the best solution?

Thank you,
Francesco