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Reverse phase separation of hemoglobin
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
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Does the acidic environment (TFA) release the heme group in hemoglobin using reverse phase HPLC. Can anyone reference a typical method using RP-LC for intact hemoglobin?
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Yes, the acidic conditions do cause the release of the heme ring. This leaves you with the individual globin polypeptide units. Those are typically separated using a C-4 column.
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