Reverse phase separation of hemoglobin

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

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Does the acidic environment (TFA) release the heme group in hemoglobin using reverse phase HPLC. Can anyone reference a typical method using RP-LC for intact hemoglobin?
Yes, the acidic conditions do cause the release of the heme ring. This leaves you with the individual globin polypeptide units. Those are typically separated using a C-4 column.
PolyLC Inc.
(410) 992-5400
aalpert@polylc.com
2 posts Page 1 of 1

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