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- Posts: 7
- Joined: Mon Jul 27, 2020 3:16 pm
I am running a RP-HPLC method for analysis of antibody, and i am seeing a big broad peak in mobile phase blank injection.
My system is UPLC, and
MPA: 0.1% TFA in Water
MPB: 0.1% TFA in Acetonitrile
Gradient:
Time Flow %B Curve
0 0.2 1 5
0.5 0.2 1 5
9 0.2 99 5
12 0.2 99 5
12.5 0.2 1 5
22 0.2 1 5
Column Temperature: 50C
Autosampler Temperature: 5C
UV: 280 nm and 214 nm.
Column of choice is Proswift RP-4H, 1x250 mm.
Here is my mobile phase injection screenshot.
https://imgur.com/a/sQTlK6K
What could i be doing wrong?