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- Posts: 16
- Joined: Wed May 25, 2022 2:42 pm
I need to analyse a sugar mixture (fructose, glucose, isomaltose, sucrose) in water by using a HILIC HPLC column but I cannot separate fructose and glucose because their peaks overlap. Are there any ideas what I could try? I use the following settings:
mobile phase: acetonitrile + water (80:20)
flow: 1 mL/min (I've already tried 0.2 mL/min and 0.5 mL/min without success)
injection volume: 1 µL (samples were diluted 1:1 with mobile phase)
column: hypersil gold amino from thermo fisher
oven temperature: 35 °C
RI detector with 35 °C; 0.5 s rise time; 5 Hz data collection rate
thank you