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- Posts: 301
- Joined: Tue Jul 12, 2016 4:47 pm
Problem is, now my Tailing has shot up to 1.4 for all actives and you can see a clear tail as the peaks elute off the column. There is no leak as I observed it for the duration of the injection, and no other changes took place (same mobile phase, washes, standard solution) so the only thing I can think of is the threads from the old inline column kit doesnt exactly line up with the column inlet, and there could be a tiny void somewhere in the connections. Even when i took it off and reconnected, same high tailing.
Any suggestions as to how to fix this, am I going to have to get a new column with a new inline filter kit to improve the tailing?