-
- Posts: 10
- Joined: Tue May 12, 2020 12:50 pm
Since I don't know what chemicals to expect, I was recommended a mobile phase:
A: Water/MeOH (95/5, v/v) + 5 mM ammonium formate + 0.1% formic acid;
B: MeOH/Water (95/5) + 5 mM ammonium formate + 0.1 % formic acid
Gradient (A/B): 95/5 --30min--> 5/95
I am not 100% convinced about the organic phase with the addition of salt. In particular, I feel that the column pressure at a flow rate of 0.3 mL/min (100x2.1x1., which is about 500 bar, is too high.
Do you use a phase with this composition?