PDA Peak extraction

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

3 posts Page 1 of 1
If you run a sample and see multiple peaks using LC-UV and want to extract one of the peaks for PDA analysis, how would you do that?
Collect the peak as it elutes out of the detector and then concentrate/reinject? That's what we used to do for peptides in my old organic lab. Collect the fraction in an Eppendorf tube and then walk it over to the mass spec.

What do you mean by PDA analysis?
If you're interested in obtaining a UV spectrum for a particular peak via extraction of PDA data, that is doable, but again - more info needed.

a PDA can collect a range of wavelengths from which people choose to extract either an entire chromatogram at particular wavelengths, or they want the whole spectrum for particular peaks. Both are pretty easily done, provided the PDA was setup properly for the collection part.
Thanks,
DR
Image
3 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry