by
natasa » Wed Jul 20, 2022 8:21 pm
Thank you for your answers.
I know that tailing is in acceptable range. I am trying to get even better peak shape.
My buffer contains from ammonium acetate with added acetic acid and fixed to pH 9,8 with triethylamine. I added acetic acid, because without it, I have problem with repeatability.
I just do not know, if maybe on HILIC column would change of pH of mobile faze (from 9,8 to about pH
make difference in peak shape? Or it could get better on other HILIC column? Now I have Kinetex HILIC (150 mm x 4,6 mm, 2,7 um). I choose HILIC column, because of good selectivity.
Maybe you have any idea of using diffrent mobile faze that could also/better work for analyse of amine on HILIC column?
According to maximum of analyte on spectro, I think that wave lenght is ok (263 nm). Injection volume is 50 uL. I also can not change sample concentration.