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- Posts: 4
- Joined: Sun Sep 12, 2021 2:36 pm
I'm trying to determine prothioconazole in commercial pesticide formulations based on CIPAC method.
Below are the gradient conditions for a Zorbax Extend C18 column
(50*4.6 (i.d)mm*3.5μm).
Time 10mM Phosphoric Acid AcN/THF/MeOH (50/25/25) Flow (ml/min)
0.0 50 50 2
2.7 50 50 2
2.71 05 95 3
3.6 05 95 3
3.61 50 50 3
4.0 50 50 2
4.4 50 50 2
I have the same column , with the same dimensions but with smaller particle size, 1.8μm.
I think that with the same gradient i will have high pressure in the system so i tried the same gradient but i double the time in each step and i reduced the flow by 1. (i.e 2ml/min to 1ml/min and 3ml/min to 2 ml/min). The peak i get was splitting.
So itried to reduce the flow more by 0.5ml/min. The peak i get was wider.
I Don't know if i'm dealing with it right.
If anyone can give me some advice.
Thanks in advance.