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- Posts: 1
- Joined: Fri Jun 17, 2022 4:21 pm
My issue, in the simplest words;
I have an Main peak that is eluting at 3.89 min. However, with the conditions of my method, the void peak comes out at 6.90 min.
NEVER have I seen something come out that fast.
YMC-Pack ODS (A) 250 mm x 4.6 mm 5um (200A pore size)
95% Ammonium Acetate pH 7.0
5% Methanol
@ 0.4ml/min flow rate
Compound is a synthetic polynucleotide
It's almost like something is pushing it through. Has anyone experience something like this? I really want to know how this is even possible.
Thank you
Charles