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- Posts: 15
- Joined: Mon Apr 11, 2022 8:09 am
I'll like some help trying to separate these two unknown compounds from ammonium fiber expansion pretreatment (AFEX) of pulp mill sludge. I ran the slurry through a couple of sizing columns and collected 10 mL fractions. A couple of the fractions are very clean (image below), but the detected unknown peaks are on top of each other. Here's the problem below (I figured out how to properly print now for better overlays comparisons):
Details:
Isocratic
Flow Rate: 0.65 mL/min
Oven Temp: 45 C
UV Cell Temp: 45 C
Column: Rezex ROA-Organic Acid 8% Cross-Linked Hydrogen
Mobile Phase: 1 mM H2SO4
The peaks don't match any of the standards in the run (about 225, sugars, organic acids, sugar alcohols, alcohols, many others). I would like to collect the fractions for LCMS but I need to separate them out first.
I tried:
- Increasing the temp from 45 to 75 in 10-degrees steps. Little change.
- Flow rate of 0.4 to 0.8 in 0.1 steps. Little change.
My next thoughts were to start playing with the mobile phase concentration, but I thought I'd ask the forum first see what anybody thought.
Thanks, y'all!!