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- Posts: 1108
- Joined: Wed Dec 22, 2010 10:17 pm
- Location: Chicago
I have read a bewildering number of papers and app notes most of them on OPA+FMOC for proline. I am aware of Waters Accutag though that is also special column plus expensive reagents. So far I haven't seen one paper where they are able to get separation of all the amino acids without a special column, the closest I have found is an Agilent App note that uses a Poroshell C18 but a special high pH column because the separation is at pH >8.
I haven't seen really anything on using FMOC alone without OPA.
I am waiting for some 3-mercaptopropionic acid for the OPA method I played with in needle derivatization and OPA (had some) and dithiothreitol (which was a bust I got 2 peaks per amino acid and a mess) and propyl mercaptan I got separation of a lot of them. Does anyone have or seen a protocol that works on a normal C18 column and separates them all?