Multidimensional wrote:RI often has VERY long equilibration times and poor sensitivity, but
IS worth a try if you have the detector.
Try this free article with tips on how to best use a RID inline.
"
HPLC Baseline Stabilization Tips for Refractive Index Detectors (RI or RID)";
https://hplctips.blogspot.com/2018/02/h ... s-for.html
I am doing a lot of work on artificial sweeteners. Steviol glysocides (working with Rebaudioside A, D, M and stevioside) and monk fruit mogroside V have a pretty poor sensitivity on UV even at 210nm, worse than the other 210 analytes potassium acesulfame, aspartame, and neotame. I can get down to 1ppm with those and in standards I get down to 10ppm with steviol glycosides running a gradient with acetonitrile and water acidified to 2.5 with H2SO4. But in samples there are all these random peaks and noise making it problematic even to get to 100ppm which are cleaned with carrez. I am planning to try RID to see if it is any better.
In my system, after the column, the line goes through the DAD then to the RID so by then the flow has probably cooled so the column compartment temperature is not relevant.