-
- Posts: 1108
- Joined: Wed Dec 22, 2010 10:17 pm
- Location: Chicago
Is it ok to go that high acetonitrile with even relative low amounts of phosphate 20mM or so? I thought going above 60% acetonitrile risks precipitation in the system.
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
Beware of the possibility for buffer precipitation when mixing it with organic solvents such as acetonitrile (ACN)—the upper limit should be 70–80% ACN. Caution particularly should be exercised in blending ACN with phosphate buffers. Limit the concentration of the phosphate buffer to <15 mM to prevent precipitation, which can cause check valve malfunctions.
Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.
Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.