How to Increase The Cost Effectiveness for HPLC Separations

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

2 posts Page 1 of 1
Matrix-rich samples often require extensive sample preparation when analyzed with HPLC and UHPLC. This is a substantial time and cost factor. A robust column material enabling a simplified sample preparation will save time and improve the lab productivity.

The performance of HPLC columns has improved dramatically in recent years, particularly in terms of separation power as measured by the number of theoretical plates per meter. The improvement in performance has been achieved primarily by a reduction in particle size.

Not all samples have benefited equally from this development: Clean samples with a relatively simple matrix are ideally suited for this new generation of columns. But for samples with a more difficult and complex matrix, the benefits are often accompanied by significant limitations. The explanation is quite simple; the smaller the particle size and the higher the sample amount injected, the more easily a column plugs or blocks. If the sample contains a matrix with colloidal and micro-particulate matter in suspension, then it becomes necessary to perform a rigorous sample “clean-up” before injection. This demands additional time for sample preparation steps, and of course involves additional cost.

The potential for cost and time savings by increasing lab productivity with faster separations and column materials providing very high matrix tolerance will be discussed.

Join our webinar on 2 December, 2021 and you will...

    Understand the challenges for matrix-rich sample analysis using HPLC and/or UHPLC and how to overcome them
    Learn how to leverage lab efficiency by simplifying the HPLC workflow using monolithic silica columns.

To register click on the link below:
https://webinar.sepscience.com/form/how ... ch-samples
David Hills
Separation Science/Chromatography Forum/
Analytical Training Solutions
david.hills@sepscience.com
thank you for giving me a better understanding of how to benefit between these patterns, I will look into it more closely. fall guys
2 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry