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- Posts: 2
- Joined: Sun Sep 12, 2021 5:52 am
How would you deal with this kind of problem? Where would you start?
I'm having trouble with the peaks I'm getting. They seem to overlay, and looked likes a two-headed peak wanting to separate. (see image 1 in this link: https://drive.google.com/file/d/1uFiJCC ... sp=sharing)
Now, I know I can work on the resolution of the 2nd and the 3rd peaks. But based on my previous runs, the peaks are single and nice in the next image. (see image 2: https://drive.google.com/file/d/1Y1vQRd ... sp=sharing)
Here's some details:
Shimadzu HPLC-20AD
Mobile Phase - 25% Methanol, 75% Phosphate Buffer
Total flow - 1.5 mL/min
column - Chromolith® SpeedROD RP-18 endcapped 50-4.6 HPLC column
I used a mixed standard of NDS isomers in the chromatograms presented.
Thank you very much.