by
Hollow » Sat Sep 04, 2021 8:12 am
should be chapter 621 (I don't have access right now)
See also FAQ:
https://www.usp.org/frequently-asked-qu ... matographybe aware to also check/optimize the extra column volume (bandspreading) of your HPLCs to keep your peaks small, otherwise the smaller particles won't show their full potential.
(e.g. use small(er) bore tubing between column and detector)
The rules for scaling/transfer of methods can be found on several locations within this forum as well as on column manufactures pages.
Also have a look at this Waters-blog article from 2015:
https://blog.waters.com/chapter-and-ver ... 21-and-you