Alternative to DNPH (2,4-Dinitrophenylhydrazine)?

[Pear]

Hey guys,

I use DNPH, as a premade solution from Sigma Aldirch in phosphoric acid, but it seems like this along with the solid powder form of DNPH is being discontinued everywhere.

I use this for analysis of aldehydes with a UV-Vis detector. Seems like DNPH is being discontinued by nearly all suppliers.

Does anybody have an alternative HPLC suitable method for detection of aldehydes?

Thanks.

[Gaetan Glauser]

A few years ago, Sobsey et al published a method for the quantification of MDA in plasma using 3-nitrophenylhydrazine (3NPH) as an alternative to DNPH. This might work for small aldehydes as well.

http://dx.doi.org/10.1016/j.jchromb.2016.07.013

[Consumer Products Guy]

Still available as DNPH powder? https://www.sigmaaldrich.com/US/en/prod ... ch/d199303

[Steve Reimer]

I see that the delivery date is projected to be next February.
I have in the past had issues with getting it shipped. Luckily no one has asked for that test for several years.

[Consumer Products Guy]

Maybe it has something to due with liability as DNPH can be explosive/dangerous.

But many chemicals are dangerous.....especially if proper precautions are not used. I imagine if gasoline or alcoholic beverages were invented today, their use would be banned....

[Steve Reimer]

The dry powder is a risk for explosions. Thus it is shipped wet.
EPA method 8315A (1996) has a recrystallization step as an appendix with no mention that it can be explosive when dry.

[James_Ball]

We were having problems with the 8315 test recently and the recrystallization step so we switched to the DNPH-HCL, corrected for molar concentration and it works great with even fewer impurities. We got it as soon as we ordered it so stock was good too, at least a couple months ago.

Don Shelly was the one who gave us that tip