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- Posts: 26
- Joined: Tue Nov 26, 2019 10:39 am
i am having problems with my pump. unsure if it is trapped air or something else.
ive purged the pump a lot, all 4 channels.
also done with isopropanol with a restriction after the pump.
tried letting it settle over the weekend, ive seen this helps before allowing the bubble to escape more easily.
but no luck. i suspect it may not be air.
i have also changed the ptfe frit. this was a little dirty. i changed it a couple of months ago - could this be a piston seal issue?
and now i cannot build pressure like before... normally i see around 90 bar with 0.5 ml/min 99/1 Aq/Org and i see only 20, with huge fluctuations.
i attached a pressure profile.i use an agilent 1260 quat pump pumping 100% aq (very low conc buffer) around 1 ml/min. pink and blank are 2 different injections