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The gradient goes from 70:30 A:B from Initial to 60 mins, 22:78 A:B at 61 mins and from 61 to 70 mins back to 70:30 A:B, all curves linear at 6. Detection wavelength is 228nm. Column temp is 35 Degrees, sample temp 21 degrees and flow rate 1ml per minute.
I set up a method to washdown the system by pumping 100% seal wash at 0.5ml per min through the system for 2 hours followed by 0.3 mls per minute of 100% Acetonitrile for 30 mins, then switch off. But the last 2 runs for the blank injections have been quite noisy and had a lot of peaks in it. Does this washdown seem appropriate for the conditions above? I'm thinking to use high aqueous to wash out the buffer from the system followed by storage in acetonitrile for 30 minutes. Everyone seems to do it differently, is there a general guide for washing down HPLC runs? Thanks in advance.