-
- Posts: 1
- Joined: Mon Jul 12, 2021 12:49 pm
Washing condition: 50:50 acetonitrile: water, column temperature- 50 degree, with gradual increase in flow rate from 0.1 ml/min to 0.4 ml/min.
Mobile phase composition for sample analysis:
Solvent A = 50:50 Acetonitrile : water with 0.2% triethylamine
Solvent B = 95:5 Acetonitrile : water with 0.2% triethylamine
Flow rate = 0.1 mL/min at column temperature 45 degree.
I am running a gradient method.
I am detecting my samples using ELSD and injection volume is 10uL.
Column details: Waters Acquity BEH Amide column of particle size 1.7um, pore size 130 Angstrom, inner diameter of 2.1 mm and length of the column is 150 mm. The guard column which I am using is Waters Vanguard pre-column of particle size 1.7um, inner diameter 2.1 mm and length is 5mm.
Kindly suggest to me regarding the high pressure issue which I am facing. Any suggestions would be greatly appreciated.
Thanks in advance!