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- Posts: 10
- Joined: Wed Jun 16, 2021 6:21 pm
I am facing some problems with unstable, noisy baseline. Photos in below link for your reference. Not sure if this is indication of presence of air bubbles? I've tried purging the flow line and autosampler several times, and equilibrating my column with initial gradient concentration for an hour or so. MP composition is 92% buffer/8% ACN, ran at flowrate 1mL/min. Pressure observed during baseline check was relatively stable, did not notice much fluctuations. Any idea how i can go about troubleshooting this? Am still new to HPLC and learning. Would appreciate any inputs! Thanks a lot!
https://ibb.co/Sch0pkQ
https://ibb.co/wS91nYb
https://ibb.co/zNZ581L