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- Posts: 3
- Joined: Wed Jun 30, 2021 1:13 pm
New here. I'm running HPLC-UV in a Gemini C18 150x2mm, 5um at 70:30 ammonium formate buffer:MeOH at 50C, FR 0.3 mL/min. I'm running stock solution isocratic, but recently there has been a huge sudden jump in my baseline when it used to just be flat. The first time it happened was at 4 minutes, and now it's happening around 13 min. The chromatography baseline actually looks flat when read at lower wavelengths. Any suggestions?
Above read at 319 nm.
Above read at 244 nm.