HPX-87H column and a guard column issue

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

3 posts Page 1 of 1
Hello!
I'm a grad student who recently started using HPLC.
This laboratory only have been used the C18 column and I have no one to ask about this hpx-87h column.

I just finished column test using organic acid standard from bio-rad and it seems okay. Then I was taking it off from the column oven to store it in the fridge. When I took the guard column KJ0-4282 then found a small plastic PEEK ferrule is stuck inside the column inlet side.

It's stuck inside so badly that I only could've removed half of it using a sharp tweezer and still there is some inside.

anyone have similar experience?
Did this happened bcz I tightened it so hard before?
Or is it bcz of something called Valco and Parker style?


And should I use a different guard column bir-rad's 125-0129 guard column as the manual says?

Any, ANY comments will be appreciated!!!
A lot to ask but no one to ask... hard time indeed
Ferrule removal tool, or even use small screw extractor from hardware store.

https://www.restek.com/en/products/acce ... rules/443/
Probably overtightened it.
In general, best to use a guard that matches the chemistry of the analytical column. Assuming you used the "carbo H+" guard from Phenomenex, that should match the HPX-87H, so I would not expect any problems on that score.
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
3 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry