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- Posts: 3
- Joined: Tue Apr 13, 2021 10:48 am
I've since tried preparing fresh mobile phase, back-flushing the column with 95% CH3CN/5% water for 20 column volumes, prepared a new blank vial - nothing seems to work.
I've also tried disconnecting the column and letting it flow directly to the PDA with a female-to-female adapter, but I don't think I can see it distinct from the solvent front (looking for the lambda max, it doesn't come up).
What else should I be looking for/checking? I've never taken apart an autosampler before, so I haven't tried to replace the needle or loop. Many thanks in advance for your help!