-
- Posts: 3
- Joined: Tue Feb 25, 2020 3:18 pm
We do not have access to rotavap and can not perform saponification but the matrix is water based so i guess saponification is not needed.
We have tried extraction with different solvents.
Energy drink:ethyl acetate
Energy drink:heptane heptane:methanol
Energy drink: isooctane Isooctane: methanol
Energy drink: butanol
But it does not seem to work, no peak for D2 is obtained. We use LCMS with APCI and C18 column. MeOH/Water as mobile phase.
Does anyone analyze ergocalciferol in similar matrix without extensive sample preparation? Or any ideas?