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- Posts: 23
- Joined: Mon Jun 03, 2013 5:56 am
We have faced an issue regarding unstable recoveries. We transferred aliquot to several HPLC vials from the SAME stock solution. However, the results obtained have an about 1% variation, while the system was stable with %RSD of 0.1% by injection of the same HPLC vial 5 times. So we assumed that there may be a cause hidden behind the procedure between solution transferring and injecting.
We have tried glass vials from agilent and waters and pp vials from thermo, and all results were unstable. I wondered if anyone had ever faced a similar issue and how to resolve it? We plan to continuously rinse a vial to see if the peak area would change after each time rinse.
Thank you in advance.
Parameters:
HPLC: Waters
Wavelength: 302nm
Mobile phase/diluent: 0.15M KH2PO4 buffer: ACN: acetic acid= 750:250:7.5