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- Posts: 6
- Joined: Wed Apr 07, 2021 4:21 pm
My diluent is 50:50 Water/Methanol with 0.01 N HCl. When I am not using 0.01 N HCl in the diluent, I am getting two separated peaks for the API (Peptide molecule). Using an acidified diluent would fix the problem.
This might very simple, but I am new to biochemistry and large molecule analysis. I know that this has to be something with the PI number of peptides (8.2 in my case) and acid/base chemistry. Can anyone suggest any reading material explaining this phenomenon?