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- Posts: 2
- Joined: Mon Sep 30, 2019 5:33 pm
We would flush the column with mobile phase (we run an isocratic run with 0.005 N sulfuric acid on a Supelcogel C-610H 9 um 30x7.8 cm column) - reverse the column and the guard column so the flow is in the opposite direction - and it would fix the problem at first. But after even a few sample injections the tailing would appear again.
I realize the dirty samples might not be helping, but any advice to keep the tailing at bay for longer? Such as good flush solvents or guard columns to help keep the system clean enough? We just bought the column 3 months ago.
Thanks so much!