-
- Posts: 1
- Joined: Wed Mar 10, 2021 4:33 pm
Column C18 with a 50:50 hexane and dichloromethane
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
2 posts
Page 1 of 1
Can I use hexane and dichloromethane as mobile phase with a c18 column ?
- tom jupille
- Site Admin
-
- Posts: 4978
- Joined: Wed Aug 11, 2004 4:55 pm
- Location: Alamo, CA / USA
You can, but that doesn't mean you should (and I don't know why you would want to).
Assuming you are using a reasonably state-of-the art column, you will void any warranty but you probably won't damage the column, but (and it's a big but!) you will have massive equilibration issues. You will have to flush the column extensively with a co-solvent (most commonly this would be isopropanol) to remove all traces of the default mobile phase/storage solvent before switching to your hexane/dichloromethane and allowing that to remove the iPrOH.
The other concern is with the HPLC system itself. It's not uncommon for manufacturers to use different seal materials for aqueous vs. non-aqueous use, so check with your vendor to confirm compatibility.
Assuming you are using a reasonably state-of-the art column, you will void any warranty but you probably won't damage the column, but (and it's a big but!) you will have massive equilibration issues. You will have to flush the column extensively with a co-solvent (most commonly this would be isopropanol) to remove all traces of the default mobile phase/storage solvent before switching to your hexane/dichloromethane and allowing that to remove the iPrOH.
The other concern is with the HPLC system itself. It's not uncommon for manufacturers to use different seal materials for aqueous vs. non-aqueous use, so check with your vendor to confirm compatibility.
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
2 posts
Page 1 of 1
Who is online
In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm
Users browsing this forum: No registered users and 1 guest
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm
Users browsing this forum: No registered users and 1 guest
Latest Blog Posts from Separation Science
Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.
Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.
- Follow us on Twitter: @Sep_Science
- Follow us on Linkedin: Separation Science