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- Posts: 9
- Joined: Tue Sep 10, 2019 11:44 pm
I am currently looking to optimize the run time for one of the main methods used where I work. So far I have gotten it down from 20-25 minutes to 14 minutes, with a chromatogram that looks like below.
[img](https://imgur.com/a/Eis7lMF)[/img]
The question I have is about the high pressure. Right now I use 2 mobile phases, A=H20 w/ 0.1%FA and B=MeOH w/ 0.1%FA. The gradient changes from 85%B - 100%B from 1min to 3min and then back down from 100%B - 85%B from 5min to 5.5min. Because of this change, the high pressure dips from about 4 min to restabilize around 12 min. This consumes the majority of my runtime, and everything elutes by 8 minutes. Is it normal for the system high pressure to have this much of a delay in restabilization after a gradient change? Is there a way to shorten this system high pressure change?