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- Posts: 68
- Joined: Wed Mar 25, 2020 8:52 am
My routine is to always flush with 50/50 MeOH/H20 in the end but when there is a shorter interruption where you intend to resume the run this seems unpractical. A possibility is to set a very low flow rather than no flow to keep the plungers moving
I don't know if I made the wrong judgement but in the future I would likely set the flow to keep plungers moving such as 10ul/min flow. It is very hard when you get interrupted to know if you will resume in two hours or the next day.
Is it likely that the 10 mM buffer in my 50/50 H20/ACN could be the cause for the loss of pump funktion 2 hours into the run, a stuck valve should cause a problem at the start? The LC method is based on the manufacturers protocol and works well in general when running samples and it is possible to dissolve 10 mM ammonium acetate in 95% ACN 5% H2O so I thought 50/50 would be safe?
I am curious whether I made the wrong judgement or if I was just unlucky? In the future I will keep plungers moving a little just to be safe.