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- Posts: 84
- Joined: Wed Oct 19, 2011 9:46 am
- Location: Montevideo, Uruguay
I want to know why this is happening.
I´ve developed a quantitation method with a Zorbax Eclipse Plus C18 RRHT column, 1,8 µm, 50 x 4.6 mm in an Infinity 1290 UHPLC. Pressure is not high, is about 230 bar. It is the only UHPLC in my lab.
So, I think, well, let´s try it in an HPLC as pressure is acceptable. Because is the only UHPLC we have, so if it brokes, HPLC may help.
When I did that, in a Shimadzu Prominence and in an Agilent 1260, A shuolder appeared. It is not the column, because another one new did the same, and so the same with mobile phase.
Solvent is HCL 0,1 N, 20 µL injection. Same happens with 15 or 10 µL.
The only thing that solves the problem, is to change solvent to water instead of HCl. So, I think pH is involved. But the question is, ¿Why this happen in HPLC but not in UHPLC?
Regards.