-
- Posts: 1
- Joined: Thu Sep 01, 2011 5:03 pm
Non-linear response in LC-MS on a glycoside of a triterpenoi
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
1 post
Page 1 of 1
In mobile phase water/acetonitrile with 0.01% HCOOH I have some response (not too good) in negative mode. I got "exponential" response with the increase in concentration of the type: 2.5, 5, 10, 20 ppm generates 1250, 5000, 20000, 80000 area count. I was expecting some ion suppression and the non-linearity to be with flattening as the concentration increases (having rather high concentrations for LC-MS). It is just the other way around. (NH4OOCH or CH3COOH in the mobile phase do not produce good signal, and nothing in positive mode). Is any way to make the response more linear?
1 post
Page 1 of 1
Who is online
In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm
Users browsing this forum: No registered users and 1 guest
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm
Users browsing this forum: No registered users and 1 guest
Latest Blog Posts from Separation Science
Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.
Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.
- Follow us on Twitter: @Sep_Science
- Follow us on Linkedin: Separation Science