negative y-intercept of the linearity
Posted: Fri Sep 18, 2020 2:16 am
Hi Experts
Who can help me out here!!
Recently, i am doing a method development for impurities test in our product by HPLC. The y-intercept of my linearity is negative. And i am pretty sure it is the bias of intercept. Some of my analyte seems disappeared in the system. Initially, i thought there might be some absorption by injection vial. but when i tried to inject different volume to the HPLC system from one vial, the y-intercept is still negative.
Then, where does the analyte go? That really bother me a lot
Thanks
Who can help me out here!!
Recently, i am doing a method development for impurities test in our product by HPLC. The y-intercept of my linearity is negative. And i am pretty sure it is the bias of intercept. Some of my analyte seems disappeared in the system. Initially, i thought there might be some absorption by injection vial. but when i tried to inject different volume to the HPLC system from one vial, the y-intercept is still negative.
Then, where does the analyte go? That really bother me a lot
Thanks