Hello everyone!

I've been dealing with this topic for a while but I couldn't find a proper answer on the internet. I work with HPLC-MS in nano. My solvents are Water and Acetonitrile.

Everytime I use it I have to run a Blank, a pull of protein sample and then I run HeLa to test if everything is going ok before running real proteomic samples. If I run HeLa right after the Blank the results obtained are not good (bad chromatogram) and I wanted to know the mechanism which the first protein sample "activates" the column and prepare it for work.

Thanks in advance.