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- Posts: 1
- Joined: Wed Sep 16, 2020 8:12 am
I've been dealing with this topic for a while but I couldn't find a proper answer on the internet. I work with HPLC-MS in nano. My solvents are Water and Acetonitrile.
Everytime I use it I have to run a Blank, a pull of protein sample and then I run HeLa to test if everything is going ok before running real proteomic samples. If I run HeLa right after the Blank the results obtained are not good (bad chromatogram) and I wanted to know the mechanism which the first protein sample "activates" the column and prepare it for work.
Thanks in advance.