Hello,

I am developing a method to analyze fructose, glucose, galactose, maltose and lactose in tobacco samples using RI detector. Wondering if anyone has experience with this? I am curious to know the best way to go about the sample preparation.

I am having some trouble finding a column that will give good chromatography for galactose. Currently, I am using Luna Omega 3um SUGAR 250 x 4.6 mm and experiencing peak tailing for galactose. I've also read about other columns not being able to separate glucose and galactose very well. Any recommendations on for a column that will seperate all 6 sugars?

For some reason, galactose and maltose have a slight shift in RT in my sample injections and I am unsure whether or not I can confirm if these peaks are truly galactose and maltose respectively. What can I do to confirm this?

The best sensitivity I can get is approximately 0.1-0.5 mg/ml across all sugars. Is this level of sensitivity reasonable for an RI detector?