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- Posts: 17
- Joined: Wed Nov 08, 2017 10:49 pm
( save proper 2D).
Think e.g. of samples with bimodal distribution of properties where common selectivity and retention is impossible to find. You can often easily top up the first column with a one of radically different selectivity and find a sweet spot for separation. There are even software approaches to help with that.
So with that why do analytical leads developing regulated methods avoid column coupling in favour of an entirely separate secondary method if a single common method cannot be found?
What is the GMP or business rationale for that?
After all this is not a proper 2D and so can be run or any standard kit really.
Robustness is of course somewhat impacted by coupling (additional variability) by is development of an entirely new, secondary method the best answer?