-
- Posts: 33
- Joined: Thu Oct 04, 2007 7:11 am
- Location: Turku, Finland
We replaced the lamp for our Shimadzu fluorescence detector (attached to Nexera serier pump system). First day the signal for tocopherols was rather constant, but slightly lower than with the old lamp. After the first batch was run, instrument was shut down for the night. Next day, the signal was suddenly approx. 10 x higher in terms of peak areas. Methods used, injection volume and the eluent (heptane) were the same.
What could be the explanation for thie sudden increase? Something to do with "running-in" of the new lamp?