by
lmh » Wed Jun 24, 2020 3:14 pm
I haven't done anything like this for years, but used to do it for carbon-14-labelled sugars with a phosphoimager as detector. I used a calibration curve from spots with known amounts of 14C. Whatever you're doing, you'd have to build an appropriate calibration curve, and check the linearity and reproducibility of your quantification (the bands are going to vary in size, so you need to know whether the intensity of the band is saturating in the middle, which will make it hard to quantify if some are more spread out than others). But yes, with lots of provisos, quantitative tlc is possible, with the right method of detection.