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- Posts: 1
- Joined: Fri May 29, 2020 9:29 am
I am going to use hplc instrumentation (RPLC to be more specific) and its first time that I am going to setup a method from the beginning .I dont have experience on that as I am graduated analytical chemist without professional experience. I plan to run a trial sample before my real one and try get familiar with my instrumentation which is a HPLC ultimate 3000 dionex. My goal in general is check the purity of my sample ( protein ) and I have to use a blank and reference standard (10μg/ml). My question is about reference std. Should I make 3 different concentrations (ex.3,5,10μg/ml) so to have 3 injections of that making a calibration curve ? my steps are not clear and I am not sure how I have to think on that. what i have until now is:
blank (1 injection)
reference std solution (10μg/ml) ,3 injections
sample