High pressure

[Paul_1002]

Hi
I noticed raising of pressure in the column during separation (usually 110 bar now around 155); except that I have peak splitting - all of my compound are flushed from the column at same time (around 3 minutes) and looks like "five peaks in the one". I changed frit on purge valve; try to clean the column with 100 ACN (my solvent was 80:20 ACN:water); I tried also equilibrate column but its still doesnt working. Can anyone give me some advice? I'm thinking about back flush of column. I'm working on Agilent system; binary pump

[dblux_]

Clogged precolumn ? (if present).

To diagnose the problem remove the column and check pressure. You will find out where pressure builds (on the column or in the system before the column).

[sga]

Replace Column with a new one. If the problem persist then try with a new column on another system.

If the problem still persists, then possibly you have made an error in your solvent preparation.

[James_Ball]

What column are you using and what are the dimensions?

It sounds like a bad contamination, but without knowing what the column material and size is and what you are injecting on it, it is difficult to trouble shoot the problem.