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- Posts: 1
- Joined: Tue May 19, 2020 8:37 pm
I have been having ongoing issues with a high RSD value for 5 consecutive injections from the same HPLC vial. Getting RSD between 1.9-2.5% (Spec <2.0%)
The mobile phase is a mixture of dioxan and water (575:400)
Run time is 40mins
Flow rate is 0.7ml/min
Injection volume 5uL
So far we have tried to eliminate the column, system, mobile phase.
The mobile phase has been re-made multiple occasions with different degassing methods/ stirring mobile phase while on system
A new column was primed and used with similar results.
The barrel of the injector was changed out on system.
A different system was used which had recently been used for other analysis with no issues.
The analysis has been carried out many times in the past with RSD of less than 1%.
Any help would be greatly appreciated.