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- Posts: 11
- Joined: Sat Sep 01, 2018 9:47 am
i am working on the WATER ACUITY UPLC BEH C18 ( 100 x 2.1mm, 1.7um) along with the mobile phase NH4OH buffer of pH 10.4 and Acetonitrile with gradient method. Column oven is having the temp. 45 Degree centigrade.
my compound of interest is actually basic in nature and also having Toluene as solvent.
I am facing the issue of column back pressure after some (approx. 20-30) injections as well as peak distortation.
Suddenly we observing that the column back pressure goes more than 1000 MPa (15000 to 16000 psi)
After so much cleaning with almost all the procedure we are not able to get the column in its lower pressure. If anyone is having some solution for this one column please suggest.