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- Posts: 4
- Joined: Tue Oct 23, 2012 6:53 am
There is some dilemma going on here.
A chemist made an API and a method needs to be developed.
MPA is 10mM ammoniumbicarbonate pH adjusted to 10 with amm. hydroxide.
MPB is Acetonitrile.
The column which is used is a Waters Xbridge C18 column, 2.1x150mm, 3.5µm
We are seeing some impurities on this used column.
Now comes the funny part:
With another used Waters Xbridge C18 column, 2.1x150mm, 3.5µm with exact same partnumber and S/N number gives the same impurities but these impurities are much lower in area.
This was analyzed with the same buffer/mobile phase just right after the first column.
Is it possible that there is some on column degradation of the compound? because there is no carry-over observed in the blanks after analyzing the API.
And why is it that some impurities are higher than on the other column?