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- Posts: 5
- Joined: Tue Jun 04, 2019 6:41 pm
Just a quick question. Would anybody envision there being an issue with using say a 50% standard reference solution in HPLC? (for quantitation, assays etc. using UV/(PDA) detectors)
Obviously it's best practice to use a high purity, well characterized, in-date primary ref standard with a CofA from a reputable company etc., but is there actually a good reason why a 50% certified solution of the same material (complete with CofA, in-date etc.) can't be used?
The rationale is that the 50% is much cheaper, obviously the dilutions for the working standard solutions will be adjusted such that the calibration curve/range match.
**Note: I would first plan of validating the 50% purity by comparison with the pure, >98% or so ref standard.
Thanks