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- Posts: 10
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Can anyone recommend a book/paper/tutorial/video on gradient optimization?
Thank you,
Mariia
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
mariia.maak wrote:
Hello! Can anyone recommend a book/paper/tutorial/video on gradient optimization? Thank you, Mariia
Consumer Products Guy wrote:mariia.maak wrote:
Hello! Can anyone recommend a book/paper/tutorial/video on gradient optimization? Thank you, Mariia
I cannot help with your question.
I will state that I got into HPLC in its early years in my R&D job, and my main general "hint" early in methods development is to use more of the stronger solvent in the gradient, and then back off to lower percentages to meet your needs.
And one "for sure" is to make the equilibration volume/time before next injection long enough, and here more is better than not enough.
Multidimensional wrote:
Suggest you try "Dry Lab" software. It is very good basic software which can be used along with some of the classic texts on HPLC (e.g. "Practical HPLC Method Development" by Snyder et al.) to learn the fundamental basics of how gradient composition changes result in changes to the chromatography. These HPLC basics are best learned by 'doing', hands-on, not reading about, but the texts and software may help you better understand the concepts and encourage you to try your own experiments to verify. Solid practical experience coupled to accurate information usually leads to faster learning.
In addition to the above, please make sure you have a solid foundation of understanding:
T0 https://hplctips.blogspot.com/2011/05/determination-of-hplc-column-dead-time.html,
K prime https://hplctips.blogspot.com/2015/06/k-prime-also-known-as-capacity-factor.html and
how delay volume effects gradient delay https://hplctips.blogspot.com/2017/02/determine-hplc-system-dwell-volume.html too.
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