Hi Deppizzymo,
To echo what everyone else is saying, the column should be completely fine if stored under the correct conditions and un-used/clean. However, before you purchase a column, in your case I think it is more necessary to find an established method. From your previous post, I would look up a USP method on the sugars you're going to analyze and then mimic the separation, methods, hardware of that of the established method. For the most part, all you need to pay attention to it the exact dimensions and specifications of the used column and their running conditions (mobile-phases, temperature, buffers, etc.) and apply that to your system/software.
In fact, on most of the manufactures' websites you can look through their application notes and almost instantly find what you're looking for in prior customer's experiences. I recall from your prior post that you are doing a particular analysis for a single customer, and I may remember it having to do with apples (I may be crazy and wrong from searching through application notes). But, you could look up carbohydrates in foods, in apples, etc. and find a method on, for example, Water's website. From there you can start to look for columns, running buffers, detection methods, etc.
I hope this provides some help! I was looking quickly for methods with an amine column, but most of the methods and application notes are for their amide or carbohydrate analysis columns.
Tyler Smith
